Column part number format capacity eqcol recommendations target applications application notes thermo scientific dionex ionpac as31 303147 2 x 250 mm 116 eq 303148 2 x 50 mm 1. First, the authors used knowledge of the sample components, particularly molecular structure information, as a guide in choosing bondedphase characteristics. For additional help choosing a column, search our chromatograms at. Comparison of achiral stationary phases based on material characteristics and the hydrophobic subtraction model. Finding an alternate or backup column that will provide acceptable selectivity and. Acdlabs column selector, column selector, chrom, tanaka parameters, quality by design, qbd, principal component analysis, pca created date. Reversed phase kinetex hplcuhplc columns from phenomenex.
Usp description functional group ymc product page l1 octadecyl silane chemically bonded to porous or nonporous silica or ceramic microparticles, 1. Comparison guide to c18 reversed phase hplc columns. Solutions for small molecule separations hplc columns in. Specifically designed for successful and reproducible method development and transfer. Comparison of achiral stationary phases based on material characteristics and the. The rate at which each sample band moves through the column depends upon the structure of the compound, the chemical structure of the stationary phase, and the column temperature. Thinlayer chromatography tlc is the traditional method of determining the. Our extensive family of products offers a variety of. Having an alternate column choice for a method reduces the risk of down time due to column problems such as a change in selectivity from one manufactured lot to another or slow supplier delivery. Siliachrom hplc column selection guide by manufacturer back to siliachrom plus hplc. Tanakaparameter based approach for chromatographic. Lc column selection column selection by usp specifications. This guide provides one of the most diverse sets of hplc phases available from any company.
Thermo scientific dionex ionpac ic column selection guide. High performance liquid chromatography hplc is a form of liquid chromatography which is used to separate the individual components of interest present in mixture andor dissolved in. Hplcuhplc column phase selection chart phenomenex uhplc. Column selection for size exclusion chromatography sec standard analysis columns column selection for size exclusion.
Pore size choose a column packing with small pore size 6080a if the solute molecular weight is less than about 2000 4000 daltons. Hplc hplc column selection by usp listing hplc column selection by usp listing recommended usp column classification phenomenex column particle shape page l1 octadecyl silane chemically bonded to porous silica or ceramic i luna c182 i spherical i 158 microparticles, 1. Oct 20, 2016 conclusion 20 best column, best mobile phase, best detection wavelength, efforts in their selection can make a world of difference while developing hplc method for routine analysis. Hplc hplc column selection by usp listing hplc column selection by usp listing recommended usp column classification phenomenex column particle shape page l1. The pump must deliver the mobile phase at high pressures between 50 and 0 bar to overcome the resistance of the stationary phase in the column. The supelco hplc column selection guide is designed to assist you in choosing the right column for your application based on the features, benefits and specifications of some of our most popular phases. Although the column is the heart of the hplc instrument and essential to its success, until now, no book has. Hplc column selection a comparison of reversedphase columns based on the widely accepted work and data of drs.
Find the right reversed phase kinetex column for your uhplc hplc method. Hplc column selection guide column parameters internal diameter an important parameter of a hplc column is the internal diameter id as this directly influences the detection sensitivity, selectivity of separation and the quantity of analyte that can be loaded onto the column. Selection of a suitable hplc column stepbystep to a suitable column depending on the separation problem since the beginning of highperformance liquid chromatography hplc in. C18 ymctriart c18 5961 ymctriart c18 exrs 62 meteoric core c18 7275. Column chromatography column chromatography is a universally used technique in chemistry laboratories in which compounds are purified from mixtures on the. At the end of this article you will find a concrete and useful application of theoretical and scientific work transformed into. The role of the mobile phase in selectivity part 4. Hplc column selection are you barking up the right tree. This guide provides direction on column selection and helps assist you through the development of your methods. To use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. Hplc columns column selection guide silica gel type reversed phase highspeed analysis conventional analysis with certificate of compliance for validation wider ph range stronger. Specialty hplc and uplc columns performance benefits and bonding performance standards application standards biosuite deae, sp, and cm hplc proteinpak hi res cm and sp uplc these series of cation and anionexchange columns include strong sp and weak cm cation as well as strong sp and weak anion deae.
Pdf column selection in high performance liquid chromatography. Conclusion 20 best column, best mobile phase, best detection wavelength, efforts in their selection can make a world of difference while developing hplc method for. Pumps for hplc and uhplc deliver mobile phase through the column that contains the stationary phase. I guess the answer to this varies widely and ive seen. High performance liquid chromatography hplc is a separation technique that uses liquid mobile phase to separate the components of a mixture. A few of the most commonly used types are described below. The column thermostat the muscles to generate heat 22 detector the organ of perception 23 detector sensitivity 25 linear range 25 data rate 26 selecting your hplc column 27 hplc mode 28 column selection basics. An indepth guide to hplc column technology highperformance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the. Optimized column dimension matched to waters lc systems. To read more about our cookies policy, please read our privacy statement. All molecules corresponding to a specific compound travel through the column at nearly the same rate, and appear as a band of molecules called the sample band.
General purpose column that provides a very high degree of analyte selectivity, especially when using low ph mobile phases. Hplc column selection guide column parameters internal diameter an important parameter of a hplc column is the internal diameter id as this directly influences the detection sensitivity. I guess the answer to this varies widely and ive seen many strategies being employed including. So, all other things being equal, it is best to start out at a low ph. We use cookies to improve your experience and our website service. Perkinelmer hplc column selection guide chromatography fundamentals the selectivity starts at one for coeluted compounds, while high values indicate better separating power.
Column selection in high performance liquid chromatography. The sample passes through the column with the mobile phase and separates in its. Hplc and uhplc columns thermo fisher scientific uk. Superior column protection for uplc, uhplc, and hplc columns and sorbents with particle sizes ranging from 1. System hplc uhplc uplc dispersion 40 l 2229 l psi column i. Column selection guide column selection guide 12 01 usp usp class no. Guide to gc column selection and optimizing separations brochure. Column selection for size exclusion chromatography sec. Silicycles alternative phases are selected based on physical and chemical similarities. Agilents zorbax column selection guide for hplc is the only chromatography guide that combines a broad offering of lc columns with expert advice that can help you achieve reliable results faster.
An ion exchange column can be either cationic or anionic. Choosing right column for reverse phase hplc separations 1. Hplc column selection hplc column selection to use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. Waters columns and analytical standards and reagents. Hplcuhplc column phase selection chart specifically designed for successful and reproducible method development and transfer ordering info use for highest performance. By tony taylor how do you choose your hplc columns. The supelco hplc column selection guide is designed to assist you in choosing the right column for your application based on the features, benefits and specifications of some of our most. Protein kw shodex tosoh phenomenex ge healthcare biorad sepax 8.
Second, they used an analysis of the separation goals in choosing column and particle physical characteristics. With over 40 years as a leader in lc column technology, you can rely on the quality of thermo scientific hplc and uhplc columns. A wide variety of hplc column types are now available for various analytical applications. Columns are the main component in hplc because the column is responsible for the separation of the sample components. If you need to operate the column at a high ph, be sure to select a column known to be stable in the ph region you choose. Tanakaparameter based approach for chromatographic column selection author. Hplc columns equivalent selection guide official gimitec. Hplcuhplc column phase selection chart specifically designed for successful and reproducible method development and transfer ordering info use for highest performance compared to fully porous media ultrahigh performance for the analysis of proteins and peptides coreshell echnolog. An efficient approach to column selection in hplc method development craig s.
Guide to gc column selection and optimizing separations. Column chromatography column chromatography is a universally used technique in chemistry laboratories in which compounds are purified from mixtures on the basis of some physicochemical property. If lowph ion suppression does not provide acceptable results, the mobile phase ph can be adjusted to help obtain the desirable separation. The current range of commercially available capillary column internal diameters enables the balancing of two factors. Agilents zorbax column selection guide for hplc is the only chromatography guide that combines a broad offering of lc columns with expert advice that can help you achieve reliable. Find the right reversed phase kinetex column for your uhplchplc method. A few of the most commonly used types are described below, classified by separation mechanism. An efficient approach to column selection in hplc method.
Determining the ideal combination of these factors assure faster delivery of desired results a validated method for separation. To find a suitable silicycle alternative to another manufacturers columns, refer to the selection guide below. Having an alternate column choice for a method reduces the risk of down time due to column problems such as a change in. Silicabased kw400 series are highperformance semimicro columns with theoretical plate number which is approximately 1. An indepth guide to hplc column technology highperformance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries. We will also discuss how separations can be further controlled and optimized by modifying mobile phase parameters, including composition, acid strength, and acid concentration. Selection of a suitable hplc column stepbystep to a suitable column depending on the separation problem since the beginning of highperformance liquid chromatography hplc in the 1960s, the method has been steadily developed and refined. At the far right, follow your final column selection to the pages indicated. Choosing right column for reverse phase hplc separations. Hplc column selection by usp listing brechbuhler ag. Figures 1 and 2 shows the effects of pore size on peak width and sample load. Tanakaparameter based approach for chromatographic column. Different types of hplc columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. Otherwise, use a column packing with 300a pore size.
Highperformance liquid chromatography hplc has become a powerful tool in the separation and isolation of biopolymers such as peptides, proteins, polynucleotides and nucleic acids. To find a suitable silicycle alternative to another manufacturers columns, refer to the selection. Usp description functional group ymc product page l1 octadecyl silane chemically bonded to porous or. High performance liquid chromatography hplc is a form of liquid chromatography which is used to separate the individual components of interest present in mixture andor dissolved in sample solution.
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